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anti phospho pdpk1 rabbit mab ser241  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti phospho pdpk1 rabbit mab ser241
    Anti Phospho Pdpk1 Rabbit Mab Ser241, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+pdk1+ser241/Phospho-PDK1+(Ser241)+Antibody/10__1007_slash_s13402___026___01300___8-69-55-61
    Average 96 stars, based on 473 article reviews
    anti phospho pdpk1 rabbit mab ser241 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Disease-specific alterations of the enteric nervous system in precancerous colonic mucosa and their implications for mucin regulation
    Article Snippet: The cells were analyzed by FACSCelesta Multicolor Flow Cytometer (BD Biosciences, Franklin Lakes, NJ).

    Cell-Signaling:

    Article Title: Atorvastatin enhances neurite outgrowth in cortical neurons in vitro via up-regulating the Akt/mTOR and Akt/GSK-3β signaling pathways
    Article Snippet: Protein concentration in the soluble fraction was measured using an Enhanced BCA protein assay kit (Beyotime Institute of Biotechnology, Haimen, China). .. Equal amounts of protein were then separated by SDS-PAGE, transferred onto nitrocellulose membranes, and probed with primary antibodies against the following proteins: rabbit anti-phospho-PDK1 (Ser241), rabbit anti-PDK1, rabbit anti-phospho-Akt (Ser473), rabbit anti-Akt, rabbit anti-phospho-PTEN (Ser380) (phosphatase and tensin homolog deleted on chromosome 10), rabbit anti-PTEN, rabbit anti-phospho-mTOR (Ser2448), rabbit anti-mTOR, rabbit anti-phospho-p70S6K (Thr389), rabbit anti-p70S6K, rabbit anti-phospho-4EBP1 (Thr37/46), anti-4EBP1, rabbit anti-phospho-GSK-3β (Ser9), and anti-GSK-3β (all from Cell Signaling Technology, Beverly, MA, USA and diluted 1:1000). .. Bound antibodies were detected with horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) (Cell Signaling Technology) each diluted 1:2000 and Supersignal West Pico chemiluminescense substrate (Pierce, Rockford, IL, USA).



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    anti phospho pdpk1 rabbit mab ser241  (Cell Signaling Technology Inc)
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    rabbit anti phospho pdk1 ser241  (Cell Signaling Technology Inc)
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    anti pdk1 phospho ser241 antibody  (Cell Signaling Technology Inc)
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    Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of <t>PDK1</t> <t>(Phospho-Ser241),</t> PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.
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    ppdk1 s241  (Cell Signaling Technology Inc)
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    Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of <t>PDK1</t> <t>(Phospho-Ser241),</t> PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.
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    Antibodies and dilutions used for Western blot analysis
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    anti phospho pdk1 ser241 rabbit monoclonal ab  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc anti phospho pdk1 ser241 rabbit monoclonal ab
    PZ-DHA inhibits <t>PDK1</t> and mTOR phosphorylation in HUVECs. HUVECs were treated with PZ, DHA, PZ-DHA (10 μM), or vehicle alone and cultured for 72 h. Cells were harvested and protein-rich cell lysates were prepared for western blot analysis of ( A ) phospho-PDK1 <t>(Ser241)/total-PDK1</t> expression or ( B ) phospho-mTOR (Ser2448)/total-mTOR expression. Equal protein loading was confirmed by β-actin expression. Data shown are mean % relative expression ± SEM from 3 independent experiments. The ANOVA multiple means comparison statistical method was performed and differences among means were compared using Tukey’s test; ** p < 0.01, *** p < 0.001. (Original Western Blot Images see ).
    Anti Phospho Pdk1 Ser241 Rabbit Monoclonal Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of PDK1 (Phospho-Ser241), PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.

    Journal: Drug Design, Development and Therapy

    Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification

    doi: 10.2147/DDDT.S513643

    Figure Lengend Snippet: Phospho-Antibody array results showing the regulatory effects of ZXF on HF. (n=6). ( A ) The overview of phospho-antibody array results. ( B ) The figure of PDK1 (Phospho-Ser241), PDK1 (Ab-241), FOXO1/3/4-pan (Phospho-Thr24/32) and FOXO1/3/4-pan (Ab-24/32). ( C and D ) Statistical results of p-PDK1/PDK1, and p-FOXO1/3/4/FOXO1/3/4. * P <0.05, ** P <0.01 vs Con group; Δ P <0.05 vs Dox group.

    Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.

    Techniques: Ab Array

    ZXF regulated the PI3K/PDK1/AKT/FoxO1 signaling pathway. (n=5). ( A ) Western blot of phosphorylated and total protein levels of PI3K, PDK1, AKT, and FOXO1. ( B – E ) Statistical results of p-PI3K/PI3K, p-PDK1/PDK1, p-AKT/AKT, and p-FOXO1/FOXO1. ** P <0.01 vs Con group; Δ P <0.05, ΔΔ P <0.01 vs Dox group.

    Journal: Drug Design, Development and Therapy

    Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification

    doi: 10.2147/DDDT.S513643

    Figure Lengend Snippet: ZXF regulated the PI3K/PDK1/AKT/FoxO1 signaling pathway. (n=5). ( A ) Western blot of phosphorylated and total protein levels of PI3K, PDK1, AKT, and FOXO1. ( B – E ) Statistical results of p-PI3K/PI3K, p-PDK1/PDK1, p-AKT/AKT, and p-FOXO1/FOXO1. ** P <0.01 vs Con group; Δ P <0.05, ΔΔ P <0.01 vs Dox group.

    Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.

    Techniques: Western Blot

    Illustration of the PI3K/PDK1/AKT/FoxO1 Signaling Pathway in Inhibiting Cardiomyocyte Apoptosis and the Role of ZXF.

    Journal: Drug Design, Development and Therapy

    Article Title: Regulatory Effects of Zhenxin Formula in Treating Doxorubicin-Induced Heart Failure: Network Pharmacology and Animal Experimental Verification

    doi: 10.2147/DDDT.S513643

    Figure Lengend Snippet: Illustration of the PI3K/PDK1/AKT/FoxO1 Signaling Pathway in Inhibiting Cardiomyocyte Apoptosis and the Role of ZXF.

    Article Snippet: Anti-Caspase-3 antibody (D3R6Y), anti-AKT1 phospho (Thr308) antibody (4056S), anti-AKT1 antibody (2920S), anti-FOXO1 phospho (Ser256) antibody (9461T), anti-FOXO1 antibody (2880T), anti-PDK1 phospho (Ser241) antibody (3438T), and anti-PDK1 antibody (5662S) were purchased from CST.

    Techniques:

    Antibodies and dilutions used for Western blot analysis

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: One day of environment-induced heat stress damages the murine myocardium

    doi: 10.1152/ajpheart.00180.2024

    Figure Lengend Snippet: Antibodies and dilutions used for Western blot analysis

    Article Snippet: Phospho-PDK1 (S241) (C49H2) , CST, No. 3438 , 1:1,000 , 1:2,000.

    Techniques: Western Blot

    Effect of environment-induced heat stress (EIHS) on mammalian target of rapamycin (mTOR) signaling. EIHS increased phosphoinositide-dependent protein kinase 1 (PDK1), phospho (p)-PDK1, p-AKT, and p-mTOR compared with thermoneutral (TN). Total AKT, total mTOR, and total p70 S6 kinase (p70S6K), phosphorylated p70S6K, total initiation factor 4E binding protein (4EBP1) and p-4EBP1 were similar between groups. Results are expressed as means ± SD. * P < 0.05; n = 8 or 9/group for PDK1 and p-PDK1. All other measures used smaller gels such that n = 6/group. One outlier was removed from TN PKD1.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: One day of environment-induced heat stress damages the murine myocardium

    doi: 10.1152/ajpheart.00180.2024

    Figure Lengend Snippet: Effect of environment-induced heat stress (EIHS) on mammalian target of rapamycin (mTOR) signaling. EIHS increased phosphoinositide-dependent protein kinase 1 (PDK1), phospho (p)-PDK1, p-AKT, and p-mTOR compared with thermoneutral (TN). Total AKT, total mTOR, and total p70 S6 kinase (p70S6K), phosphorylated p70S6K, total initiation factor 4E binding protein (4EBP1) and p-4EBP1 were similar between groups. Results are expressed as means ± SD. * P < 0.05; n = 8 or 9/group for PDK1 and p-PDK1. All other measures used smaller gels such that n = 6/group. One outlier was removed from TN PKD1.

    Article Snippet: Phospho-PDK1 (S241) (C49H2) , CST, No. 3438 , 1:1,000 , 1:2,000.

    Techniques: Binding Assay

    PZ-DHA inhibits PDK1 and mTOR phosphorylation in HUVECs. HUVECs were treated with PZ, DHA, PZ-DHA (10 μM), or vehicle alone and cultured for 72 h. Cells were harvested and protein-rich cell lysates were prepared for western blot analysis of ( A ) phospho-PDK1 (Ser241)/total-PDK1 expression or ( B ) phospho-mTOR (Ser2448)/total-mTOR expression. Equal protein loading was confirmed by β-actin expression. Data shown are mean % relative expression ± SEM from 3 independent experiments. The ANOVA multiple means comparison statistical method was performed and differences among means were compared using Tukey’s test; ** p < 0.01, *** p < 0.001. (Original Western Blot Images see ).

    Journal: Biomolecules

    Article Title: Phloridzin Docosahexaenoate, an Omega-3 Fatty Acid Ester of a Flavonoid Precursor, Inhibits Angiogenesis by Suppressing Endothelial Cell Proliferation, Migration, and Differentiation

    doi: 10.3390/biom14070769

    Figure Lengend Snippet: PZ-DHA inhibits PDK1 and mTOR phosphorylation in HUVECs. HUVECs were treated with PZ, DHA, PZ-DHA (10 μM), or vehicle alone and cultured for 72 h. Cells were harvested and protein-rich cell lysates were prepared for western blot analysis of ( A ) phospho-PDK1 (Ser241)/total-PDK1 expression or ( B ) phospho-mTOR (Ser2448)/total-mTOR expression. Equal protein loading was confirmed by β-actin expression. Data shown are mean % relative expression ± SEM from 3 independent experiments. The ANOVA multiple means comparison statistical method was performed and differences among means were compared using Tukey’s test; ** p < 0.01, *** p < 0.001. (Original Western Blot Images see ).

    Article Snippet: Anti-CDK4 rabbit monoclonal Ab, anti-cyclinD3 rabbit monoclonal Ab, anti-total- PDK1 rabbit monoclonal antibody (Ab), anti-phospho-PDK1 (Ser241) rabbit monoclonal Ab, anti-total-mTOR rabbit monoclonal Ab, anti-phospho-mTOR (Ser2448) rabbit monoclonal Ab, anti-RhoA rabbit monoclonal Ab, anti-Rac1/2/3 rabbit monoclonal Ab, anti-Cdc42 rabbit monoclonal Ab, HRP-conjugated rabbit anti-β-actin, and HRP-conjugated donkey anti-rabbit Ab were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA).

    Techniques: Phospho-proteomics, Cell Culture, Western Blot, Expressing, Comparison